Coding

Part:BBa_K2929002

Designed by: James Hammond   Group: iGEM19_St_Andrews   (2019-10-10)


Human IgE CH3 Domain

CH3 Domain of the Human IgE antibody. Codons optimised for Escherichia coli.

5' NcoI and 3' BamHI cut sites.

Usage and Biology

The Human IgE CH3 Domain expresses well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.

Figure 1. Human IgE wild-type expression, showing clear bands corresponding to insoluble recombinant protein for the induced fraction. Soluble recombinant protein expression is not visible on this gel.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 334
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//proteindomain
Parameters
protein